| 1. | Fghrl and zfghrl : we failed to find out the 5 " ends nuclear acids due to a incomplete genome sequences . the fghrl consists of 1755bp and encodes a 584 amino acid protein
东方?和斑马鱼ghr1 ：由于东方?基因组测序工作没有完成的原因，本研究没有分离出fghr1cdna5 ’末端的部分。 |
| 2. | The complete human znf323 cdna sequence is 3179bp and contains a 1221 - bp open reading frame ( orf ) that encodes a 407 amino acid protein with a scan domain and 6 zinc finger c2i12 motifs
Znf323长3179个碱基，含有一个长为1221个碱基的开放阅读框，编码一个长为407个氨基酸的蛋白质，它包含一个scan框和6个c2h2型锌指。 |
| 3. | The zfp28 cdna sequence is 4104 bp and contains a 2076 bp orf that encodes and 868 amino acids protein with an n - terminal signal peptide , two krab domain , and 14 c - termianal c2h2 zinc finger motifs
Zfp28基因长4076个碱基，含8个外显子，在基因组dna上长18kb 。它编码的蛋白质全长868个氨基酸，含1个信号肽， 2个krab框和14个c2h2型的锌指。 |
| 4. | 2 the polymorphism of ul136 gene dna and amino acid sequence 1 ) the length of ul136 orf in all 18 clinical isolates was similar to that of toledo , 723 bp in size . they hade the potential to encode 241 amino acid protein
? 2 ?二月136基因编码区及编码产物氨基酸序列的多态性株临床低传代分离株ul136orf长度均与toedo株相同，为723hp ，预测编码241个氨基酸的蛋白。 |
| 5. | The complete human znf359 cdna sequence is 3270 bp and contains a 1932 bp open reading frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs
Znf359基因长3271个碱基，含6个外显子，在基因组dna上长18kb 。它编码的蛋白质全长643个氨基酸，含一个krab框， 16个c2h2型的锌指，在两者之间还有一个典型的中间重复序列。 |
| 6. | Two positive clones were sequenced , and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii , bamh i and bgi ii , this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys , x is serine , valine , ilistidine or lysine
结果显示：扩增的cdna片段长度为289bp ，其中含有一个编码45个氨基酸的开放阅读框，阅读框所编码的氨基酸中含有10个半胱氨酸，且在序列中均排列成cys - x - cys ，其中x为ser 、 val 、 his或lys 。这些特征说明扩增的基因片段为家蝇mt基因序列的一部分。此基因序列片段与果蝇mtn基因序列的同源性达到85 . 0 ，扩增的基因序列中含有三个内切酶位点bg 、 bam和bg ，这一点也和果蝇mtn基因十分相似。 |
| 7. | The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs . the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs . the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide , two krab domains and 14 c - terminal c2h2 zinc finger motifs
Znf325长2697个碱基，含有一个长为1389个碱基的开放阅读框，编码一个长为462个氨基酸的蛋白质，它包含15个c2h2型锌指结构； znf359长3270个碱基，含有一个长为1932个碱基的开放阅读框，编码一个长为643个氨基酸的蛋白质，它包含一个n -端krab结构域和16个c -端c2h2型锌指结构； zfp28长4104个碱基，含有一个长为2076个碱基的开放阅读框，编码一个长为868个氨基酸的蛋白质，它包含一个n -端信号肽，两个krab结构域和14个c -端c2h2型锌指结构。 |
| 8. | The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53 . 484 kda and an isoelectric point of 6 . 75 . at the primary sequence level , it shared high homology with clr , so was named clr - like serine protease , clsp . the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )
该蛋白在核酸和氨基酸水平上与人补体组分cl :表现高度同源，故将其命名为补体cl :样丝氨酸蛋白酶(旦lr一like旦erineprotease ， clsp ) ，该基因已经在ge心ank登录(登录号为af17s985 ) 。 |
| 9. | Layered and pillared material are a kind of multifunctional material which were developed in recent years , much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity , nonlinear optics and physic . a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ) , different guest molecules inserted into a - zrp have been studied in detail , those guest molecules include amine , alcohok amino acid protein , enzyme coornadiate compound and coronal compound . the intercalation guest is restricted by their size and basicity
层柱材料是近年来发展起来的一类多功能材料，由于其在离子交换、催化、固态质子导体、非线性光学以及医学等方面的广泛应用而受到国内外研究者的重视，大量文献报道了-磷酸氢锆zr ( hpo _ 4 ) _ 2 ? h _ 2o ( - zirconiumphosphate ，缩写为- zrp )的超分子插层化合物及插层性能，其中对不同的客体分子对磷酸锆的嵌入做了详细的报道，客体分子的种类包括氨、醇、氨基酸、蛋白质、酶、配合物、冠状化合物等。 |
| 10. | Its gene includes seven exons ( 3 - 9 ) and six introns ( 3 - 8 ) . with respect to zfghrl , it is also like other ghr1s , which consists of 1578bp and encode a 528 amino acid protein . its gene only includes eight exons ( 1 - 5 , 8 ) and seven introns ( l - 5 ) . various methods used to find out the transmembrane and boxl domain of zfghrl but the result turns out to be fruitless due to unknown reason . fghr2 and zfghr2 : fghr2 includes eight exons and seven introns , which consists of 1710 bp and encodes a 569 amino acid protein ; zfghr2 includes eight exons ( 1 - 5 , 8 ) and seven introns ( 1 - 5 ) , which consists of 1758 bp and encodes a 484 amino acid protein
得到的fghr1含有7个外显子( 3 - 9 )和6个内含子( 3 - 8 ) ，由1755个bp的核苷酸组成并编码一个584个氨基酸残基的蛋白质：得到的zfghr1包含有1587个核苷酸并编码一个528个氨基酸残基的蛋白质，理学硕士学位论文:南方贴生长激素受体cdna的分子克隆和鱼类存在两种生长激素受体的证明其基因只有8个外显子( l一5 ， 8 )和7个内含子( 1一5 ) 。 |
